food grade high temp alpha amylase

Detail Information

High-temperature Alpha-amylase

High-temperature Alpha-amylase is obtained by liquid submerged fermentation and extraction of Bacillus licheniformis.
It has excellent thermostability, which is widely used in the industry of alcohol, beer, vinegar,
soy sauce, textile, printing, dyeing, papermaking and other fermentation fields.

Definition of Activity

One unit of enzyme activity is marked as U/g or U/mL, and defined as one gram solid enzyme powder
or one milliliter liquid enzyme liquefies one gram soluble starch in one hour at 70℃ and pH6.0.

Product Characteristics

 

  1. Optimum operation temperature: 55℃-95℃;
  2. Optimum operation pH: 4.5-8;

Graph1 High-temperature Alpha-amylase Temperature Curve

 

Graph2 High-temperature Alpha-amylase pH Curve

 

  1. Good stability and adapted to different reaction environment;

Graph3 High-temperature Alpha-amylase Stability Curve

 

  1. No need too much Ca2 +, 50-70 ppm Ca2 + is enough.

 

 

Table1. The Effect of Metal Ion on the activity of High-temperature Alpha-amylase

Metal IonConcentration C/mol.L-1Relative activity/%Evaluation
Ba2+

Cu2+

Li+

Ca2+

Mg2+

Mn2+

Fe2+

Al3+

Zn2+

Comparation

10-3

10-3

10-3

10-3

10-3

10-3

10-3

10-3

10-3

/

113.35

117.43

102.07

126.65

104.07

111.98

87.39

83.45

60.42

100.00

+

+

0

+

0

+

0

Note: + refers to activation; – refers to inhibition; 0 refers to no obvious impact (the effect on enzyme activity is within 10%)

 

Product Application

 

1.In the process of brewing, mix the accessories in mash copper (corn starch, rice, barley, etc.)
with water as the mixing ratio 1:3-5, then add enzyme (0.3 liter 20,000 U/g enzyme per ton auxiliary materials),
rise temperature up to 92-92 ℃ for 20 to 30 minutes, rise temperature up to 100 ℃ for heat preservation
when the color of  iodine test changes to reddish-brown;

  1. In the alcohol production, add enzyme (0.3 liter 20,000 U/g enzyme to per ton raw material,
    pH 6.0-6.5, stir then send into the digester pump or continuous cooking heater,
    control temperature to 100 ±5 ℃for 100 minutes, then saccharification after cooling;
  2. In the applications in monosodium glutamate, starch sugar industry, adjusting the pH to 6.0-6.5,
    add enzyme (0.6 liter 40,000 U/g enzyme to per ton raw material. If using interval liquefaction,
    can rapidly heat up to 100±5 ℃in LNG tank, keep in 95-100 ℃for heat preservation more than 30 minutes;
    If using spray liquefaction, spray with temperature about 105 ℃,
    keep in 95 ℃for heat preservation more than 60-120 minutes.

 

4.In enzymatic desizing process, the dosage of enzyme is related with equipment, process,
fabric structure and amount of sizing ect., small sample test can find optimal amount of enzyme,
Recommended optimal amount of enzyme (20000 U/g) is as follow as the test starting point :
Impregnation method of usage: 2.0 to 6.0 g/L(ml/L), under the condition of processing of pH6.0-7.0,
temperature 85-95 ℃ for 20 to 40 min;Continuous steam usage: 4.0 to 10.0 g/L (ml/L),
under the condition of processing of pH6.0-7.0,  temperature 95-105 ℃for 10-15 min.

 

Specification and Usage

NameHigh-temperature Alpha-amylase
Activity40,000U/g(mL)
TypePowder, Liquid
UsageThe actual dosage and using method should be according to the actual using condition of the trial.

 

Package and Storage

Packaging: Solid products: 25kg/Kraft-bag or 20kg/Barrel; Liquid products: 30kg/Plastic barrel or

225kg/Plastic barrel.

Store in original package under sealed, room temperature condition for 12 months to solid form,
and 6 months to liquid form (less than 25 ℃).

Avoid to be exposed to the sun, rain, high temperature and high humidity.

 

 

Technical Service

Offer high-temperature alpha-amylase usage formula, enzyme activity test and related product technical service to ensure the result.

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